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The Effect of Manganese on digestive enzymes and intestinal histology in Reared Young Beluga (Huso huso)
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Fatemeh Hemmati   , Hossein Khara * , Habib Vahaabzadeh Roudsari , Rezvanollah Kazemi |
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Abstract: (773 Views) |
Introduction: This research aims to determine the effect of different amounts of dietary manganese on digestive enzymes and intestinal tissue in rearing young beluga (Huso huso).
Materials & Methods: For this purpose, 180 pieces of beluga were selected (respectively, with an average weight of 266±3.05 grams and a total length of 38.23±0.49 cm). In this study, six treatments and each treatment with three repetitions, with concentrations of 5 (Mn1), 10 (Mn2), 15 (Mn3), 20 (Mn4) and 25 (Mn5) mg of manganese sulfate monohydrate (MnSO4H2O) in each kilogram of diet and control (Mn0) without adding MnSO4 supplement was carried out at Dr. Beheshti's Sturgeon Breeding and Regeneration Center, Sanger Dam, Rasht city, for two months. After weighing, the desired amount of supplement was added to the factory powdered food along with deionized water. The fish were biometrically measured at the end of each month. Physical and chemical factors of water (temperature, oxygen and pH) were measured and recorded daily. At the end of the experiment, three pieces of fish were selected from each repetition and blood was taken from the caudal vein to measure digestive enzymes. Also, samples were taken from different parts of the intestine (anterior, middle and posterior) for histological studies.
Results: The results of the indices of digestive enzymes showed that the amount of amylase, lipase, and protease enzymes had a significant difference between some of the experimental treatments and the control group (p<0.05) and the maximum and minimum of these enzymes were observed in Mn2 and Mn5 treatments, respectively. In tissue studies, symptoms such as bleeding, hyperemia, necrosis, vacuolation were observed in different parts of the intestinal tissue in all treatments, even the control treatment, but the best intestinal tissue condition was in the Mn3 treatment.
Conclusion: According to the results of this study, the short-term addition of 10-15 mg of manganese per kilogram of diet of bluga improves the activity of digestive enzymes and also reduces intestinal tissue damage. |
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| Keywords: Beluga (Huso huso), Dietry, Manganese sulfate, Digestive enzymes, Intestinal histology |
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Full-Text [PDF 1130 kb]
(266 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2025/10/9 | Accepted: 2025/09/1 | Published: 2025/12/2
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