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Evaluation of polymorphism of IGFBP3 gene and its association with growth and carcass traits in Mazandaran native rooster
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Hossein Attarchi Dr. *   , Mojtaba Tahmoorespoor , Mojtaba Ahani Azari Dr. , Mokhtar Mohajer Dr. |
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Abstract: (93 Views) |
Introduction: This study aimed to study polymorphism of the exon 1 region of the IGFBP3 gene and its association with growth and carcass traits in Mazandaran native rooster.
Materials & methods: For this purpose, 200 male chicks of Mazandaran native fowls reared in the same conditions and all were slaughtered at 12 weeks of age, were used. Traits before and after slaughter were measured and recorded. Before the destruction of all birds, blood sample and extraction of DNA from samples were performed by Cinnagen kit. Then, the desired locus of gene was amplified using specific primers and genotyped using PCR-RFLP method by specific enzyme. Phenotypic and genotypic data analysis conducted using statistical software of SAS9.2.
Results: Phenotypic and genotypic data analysis conducted using statistical software of SAS9.2. The frequency of each allele (+) and (-) were estimated to be 0.805 and 0.195 in IGFBP3 locus, respectively. Hardy-Weinberg equilibrium study using Chi-square test showed that the study population in loci is not in balance. The results showed a significant association between IGFBP3 gene genotypes and the traits of live weight at 8 weeks and abdominal fat weight (P<0.05).
Conclusion: Based on the results of this research can be concluded that IGFBP3 gene can be used as a candidate for growth and carcass traits in Mazandaran native rooster breeding programs. |
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| Keywords: Polymorphism, Native rooster, Gene, Growth traits, Carcass, IGFBP3 |
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Full-Text [PDF 650 kb]
(32 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2026/08/11 | Accepted: 2025/04/30 | Published: 2025/04/30
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