fa بررسی بیان ژن گیرنده آندروژن در پاسخ به تغییرات تراکم شکارچی و تراکم نر در ماهی گوپی (Poecilia reticulata) تخصصي Special پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">&nbsp;</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">ماهی گوپی به دلیل داشتن طیف گسترده‌ای از رفتارهای تولیدمثل و وجود منابع ژنتیکی و ژنومی یک</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">گونه مدل برای مطالعه تکامل رفتار تولیدمثل می‌باشد. آندروژن‌ها هورمون تنظیم</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">کننده اولیه اسپرماتوژنزیز هستند و برای</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">کنترل</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">عملکرد تولیدمثل در مراحل مختلف رشد توسط گیرنده آندروژن به سلول متصل می‌شود. با توجه به اهمیت هورمون آندروژن در فرایند تولیدمثل، پژوهش حاضر به</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">منظور بررسی بیان ژن گیرنده آندروژن در مواجهه با تراکم مختلف شکارچی و تراکم متفاوت ماهی گوپی نر انجام گرفت.</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black"> نتایج این تحقیق بینش‌هایی را در مورد این</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">که چگونه تراکم ماهی‌های شکارچی و گوپی نر بر بیان ژن گیرنده آندروژن تأثیر می‌گذارد وهم</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">چنین تأثیر متقابل بین عوامل محیطی و تنظیم تولید‌مثل در این گونه مدل را ارائه می‌کند.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">مواد و روش</span></span></span></span></b><b> </b><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">ها:</span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black"> نمونه</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">برداری از ماهیان نر و ماده گوپی پس 5 دقیقه، 5 ساعت، 5 روز و 15 روز پس از مواجهه با گونه شکارچی صورت گرفت. نمونه‌ها بلافاصله در تانک ازت مایع قرار گرفته و تا زمان استخراج </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:black">RNA</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black"> در یخچال 80- نگه</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">داری شدند.</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">داده‌های مربوط به ژن هدف به</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">وسیله ژن رفرنس </span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:black">s</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">18 با روش محاسباتی</span></span></span></span><span dir="LTR" new="" roman="" style="font-family:" times="">2^(-∆∆ct) </span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">&nbsp;تحلیل شد.</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">در ادامه نرمال بودن داده‌ها و آنالیز آماری دوطرفه جهت معنی‌دار بودن اثر تیمارها صورت گرفت.</span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">نتایج:</span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black"> بیان ژن مورد نظر نشان داد که اثر تراکم مختلف شکارچی در زمان‌های متفاوت مواجهه با گونه شکارچی، اثر معنی</span></span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">داری در میزان بیان ژن گیرنده آندروژن داشته است (0/05</span></span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times=""><span style="color:black">P<</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span b="" baran="" style="font-family:"><span style="color:black">). </span></span></span></span></span></span></span></span></span><br> <b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">بحث و نتیجه</span></span></span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">گیری:</span></span></span></span></span></b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="background:white"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black"> بیان ژن گیرنده آندروژن با حضور شکارچی کاهش یافته و این تغییر موجب کاهش رفتار تولیدمثلی در گوپی‌ها می‌شود.</span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Introduction:</span></span></b> <span style="font-family:&quot;Times New Roman&quot;,serif">Due to its broad range of reproductive behaviors and availability of genetic and genomic resources, the guppy fish&nbsp;is a model species for studying the evolution of reproductive behavior. Androgens are the primary hormones regulating spermatogenesis and bind to androgen receptors on cells to control reproductive function across different growth stages. Given the importance of androgens in reproductive processes, the present study aims to examine the expression of the androgen receptor gene in response to varying predator densities and different male guppy densities. The findings of this research offer insights into how predator and male guppy densities affect androgen receptor gene expression, as well as the interaction between environmental factors and reproductive regulation in this model species.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Materials & Methods:</span></span></b> <span style="font-family:&quot;Times New Roman&quot;,serif">Sampling of male and female guppies was conducted 5 minutes, 5 hours, 5 days, and 15 days after exposure to predators. The samples were immediately placed in liquid nitrogen tanks and stored at -80&deg;C until RNA extraction. The data related to the target gene were analyzed using the comparative method 2^(-∆∆ct) with the s18 reference gene. Subsequently, the normality of the data and two-sided statistical analysis were performed to determine the significant effects of treatments.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Results:</span></span></b> <span style="font-family:&quot;Times New Roman&quot;,serif">The expression of the target gene indicated that varying predator densities at different exposure times had a significant effect on androgen receptor gene expression levels (P<0.05). </span></span></span></span><br> <b><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Conclusion:</span></span></span></span></b> <span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,serif">Androgen receptor gene expression decreased in the presence of predators, leading to a reduction in reproductive behavior in guppies.</span></span></span></div> ماهی گوپی, گیرنده آندروژن, شکارچی, تولید‌مثل, بیان ژن Guppy fish, Androgen receptor, Predator, Reproduction, Gene expression 25 30 http://journalaer.com/browse.php?a_code=A-10-2-49&slc_lang=fa&sid=1 Mahdi Katebi مهدی کاتبی 10031947532846001203 10031947532846001203 No Amir Reza Abed-Elmdoust امیرضا عابد علم‌دوست amirabed@ut.ac.ir 10031947532846001204 10031947532846001204 Yes Hamid Farahmand حمید فرحمند 10031947532846001205 10031947532846001205 No Ali Momeninejad علی مومنی نژاد 10031947532846001206 10031947532846001206 No